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Y3627

Millipore

Yeast Carbon Base

suitable for microbiology, NutriSelect® Basic

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

application(s)

clinical testing
food and beverages
life science and biopharma
microbiology

storage temp.

2-8°C

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Related Categories

Application

Recommended for use in classification of yeasts on the basis of their ability to assimilate nitrogen compounds.

Components

Ingredients (g/L)
Dextrose, 10.00
L-Histidine hydrochloride, 0.001
DL-Methionine, 0.002
DL-Tryptophan, 0.002
Biotin, 0.000002
Calcium pantothenate, 0.0004
Folic acid, 0.000002
Inositol, 0.002
Niacin, 0.0004
p-Amino benzoic acid, 0.0002
Pyridoxine hydrochloride, 0.0004
Riboflavin (Vitamin B2), 0.0002
Thiamine hydrochloride, 0.0004
Boric acid, 0.0005
Coppers sulfate, 0.00004
Potassium iodide, 0.0001
Ferric chloride, 0.0002
Manganese sulfate, 0.0004
Sodium molybdate, 0.0002
Zinc sulfate, 0.0004
Monopotassium phosphate, 1.00
Magnesium sulfate, 0.50
Sodium chloride, 0.10
Calcium chloride, 0.10

Preparation Note

A. Nitrogen Assimilation test
Prepare the broth base at 10 x concentration by dissolving 11.7 grams of Yeast Carbon Base in 100 mL of distilled water. Add the appropriate nitrogen source. Warm if necessary to dissolve the medium completely. Sterilize by filtration.
B. Detection of yeasts, other than Saccharomyces cerevisae
Dissolve 2.35 grams of Yeast Carbon Base in 100 mL of distilled water.
C. Detection of wild yeasts in beer and other brewing components
Add 0.33 g of ammonium sulphate (Catalog No. A2939) and 4 g of bacteriological agar (Catalog No. A5306) to base B. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Culture Method for Beer Quality Control

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Protocols

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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