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  • Interference by naturally occurring fatty acids in a noncellular enzyme-based aromatase bioassay.

Interference by naturally occurring fatty acids in a noncellular enzyme-based aromatase bioassay.

Journal of natural products (2006-04-29)
Marcy J Balunas, Bin Su, Serena Landini, Robert W Brueggemeier, A Douglas Kinghorn
ABSTRACT

Natural product drug discovery efforts frequently utilize noncellular screening assays. Fatty acids are commonly found in natural product extracts, and some have been shown to interfere with noncellular assays. Several pure fatty acids were tested using a noncellular aromatase assay, with the unsaturated analogues showing strong inhibitory activity, while the saturated analogues were inactive. Unsaturated fatty acids were further tested against SK-BR-3 hormone-independent human breast cancer cells that overexpress aromatase and were found to be inactive. In natural product screening efforts, especially using plant seeds, it is recommended that extracts active in noncellular bioassays should be dereplicated for the presence of fatty acids prior to bioassay-guided fractionation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Linolenic acid, ≥99%
Sigma-Aldrich
all-cis-7,10,13,16,19-Docosapentaenoic acid, synthetic, ≥97%
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Myristic acid, Sigma Grade, ≥99%
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Linoleic acid, liquid, BioReagent, suitable for cell culture
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Linoleic acid, ≥99%
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Palmitic acid, BioXtra, ≥99%
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Linolenic acid, ~70% (GC)
Supelco
Linolenic acid, analytical standard
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Myristic acid, analytical standard
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Palmitic acid, ≥98% palmitic acid basis (GC)
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Linoleic acid, technical, 58-74% (GC)
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Linoleic acid, analytical standard
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Palmitic acid, ≥98%, FCC, FG
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Myristic acid, ≥95%, FCC, FG
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Myristic acid, natural, ≥98.5%, FG
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Myristic acid, ≥98.0% (GC)
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Stearic acid 50, tested according to Ph. Eur.
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Palmitic acid, ≥99%
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Palmitic acid, analytical standard
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Oleic acid, Selectophore, ≥99%
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Arachidonic acid, from non-animal source, ≥98.5% (GC)
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Pentadecanoic acid, 99%
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Stearic acid, ≥95%, FCC, FG
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cis-5,8,11,14,17-Eicosapentaenoic acid, analytical standard
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Oleic acid, technical grade, 90%
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cis-5,8,11,14,17-Eicosapentaenoic acid, ≥99%