1.46597
PPLO Broth
bottle of, ready-to-use, bottle volume 500 mL , filling volume
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About This Item
UNSPSC Code:
12352201
Recommended Products
Agency
EP 2.6.1
JP 4.06
USP 71
71
Quality Level
sterility
sterile; autoclaved
form
liquid
feature
closure type screw cap with 3 septa
ready-to-use
packaging
bottle of
technique(s)
direct inoculation: suitable
bottle capacity
1000 mL
bottle volume
500 mL , filling volume
pH
7.8± 0.2
General description
PPLO Broth is a nutrient rich mycoplasma detection media. Mycoplasmas are the smallest bacteria besides nanobacteria. Due to the lack of cell wall structures they pass filters with pore sizes of 0.2 μm and show a great sensitivity towards osmotic changes within their habitats. They grow aerobically or under facultative anaerobic conditions.
This medium contains beef heart infusion and peptone to supply nutrients required for the growth of mycoplasmas. Sodium chloride maintains osmotic equilibrium. Yeast extract supplies a variety of B-complex vitamins and enhances growth. Normal horse serum provides a protein source. Penicillin and thallium acetate are added as inhibitors of bacteria. Yeasts and molds are inhibited by the amphotericin B contained in the medium.
This medium contains beef heart infusion and peptone to supply nutrients required for the growth of mycoplasmas. Sodium chloride maintains osmotic equilibrium. Yeast extract supplies a variety of B-complex vitamins and enhances growth. Normal horse serum provides a protein source. Penicillin and thallium acetate are added as inhibitors of bacteria. Yeasts and molds are inhibited by the amphotericin B contained in the medium.
Application
PPLO broth is used to detect presence of mycoplasma in test samples through direct inoculation or sterile filtration methods.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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