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MAIPNTR10

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, non-sterile

Synonym(s):

96-Well PVDF Plate, Hydrophobic 96-Well Plate, PVDF 96-Well Plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

description

This clear 96 well filter plate has a Hydrophobic PVDF membrane used for PAMPA.

sterility

non-sterile

product line

MultiScreen®

feature

hydrophobic
lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

parallel artificial membrane permeation assay (PAMPA): suitable

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

binding type

high binding surface

shipped in

ambient

General description

Filter Code: IP
Increasing drug candidate predictability earlier in the discovery pipeline using non-cell-based assays can improve candidate throughput during the ADME stage. Non-cell-based permeability assays are predictive models for oral absorption of development candidates.

MultiScreen Permeability, MultiScreen-IP, and MultiScreen Acceptor plates support artificial membrane strategies for predicting passive transport.

Membranes have uniform pore structure and density for reliable transport:
- Individual wells minimize candidate cross-talk
- Compatibility with automated liquid handling systems increases throughput

Permeability Assay:
- The MultiScreen Permeability plate has a polycarbonate membrane, which supports a hexane/hexadecane artificial layer for permeability assays.1

PAMPA Assay:
- The MultiScreen-IP plate has a hydrophobic PVDF membrane, which supports a lipid bilayer for PAMPA asays.2,3

MultiScreen Acceptor Plate:
- The MultiScreen Acceptor plate can be used with either plate to capture passively transported compounds. Constructed of 100% PTFE, the plate can improve LC/MS results depending on the charge of the small molecules being evaluated.

Application

Research Category
Assays

Protein

Physical form

Color Code: Clear

Other Notes

Automation Compatible: No

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes

Combining solubility & PAMPA assays streamlines drug permeability testing.

Protocols

Assays that predict passive absorption of orally administered drugs have become increasingly important in the drug discovery process. As previously described by Faller and Kansy such assays provide rapid, low cost and automation friendly methods to measure a compound’s passive permeability.

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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