SRE0096

Bovine Serum Albumin

≥98.0%, New Zealand Origin, ≤3 EU/mg endotoxin, protein quantification

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• MDL number: MFCD00130384
• UNSPSC Code: 41141801

Properties

• product name: Bovine Serum Albumin, heat shock fraction, protease free, IgG free, low endotoxin, Ph 7.0, ≥98%
• grade: standard grade
• Assay: ≥98.0%
• form: powder
• mol wt: ~66 kDa
• purified by: heat shock fractionation
• origin: New Zealand origin
• availability: available only in China
• technique(s): protein quantification: suitable
• impurities: ≤3 EU/mg endotoxin
• pH: 7.0
• cation traces: heavy metals (as Pb): ≤10 ppm
• foreign activity: Ribonuclease, none detected; endonuclease, none detected; protease ≤0.005 units/mg solid; proteinase, none detected
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable