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C-12071

Sigma-Aldrich

Human Follicle Dermal Papilla Cells (HFDPC)

500,000 cryopreserved cells

Synonym(s):

HFDPC cells

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human dermis

packaging

pkg of 500,000 cells

morphology

( dermal)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Skin

Application

Primary Human Follicle Dermal Papilla Cells (HFDPC) are isolated mainly from human dermis of the scalp, originating from the occipital or temple region and are provided in a cryopreserved format. Information on donor hair and skin color is available on request. HFDPC stain positive for alkaline phosphatase.Dermal papillae are embedded in a laminin and collagen IV rich extracellular matrix at the base of the hair follicles. They are essential for the induction and maintenance of hair growth. Since they have androgen receptors, they can be used for in vitro screening of androgen blocking reagents.

Quality

Rigid quality control tests are performed for each lot of Follicle Dermal Papilla Cells. They are tested for cell morphology, adherence rate, and cell viability. Immunohistochemical tests for significant markers, e.g. alkaline phosphatase, are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Alkaline phosphatase positiveGuaranteed population doublings: >10

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Primary Human Follicle Dermal Papilla Cells and Growth Media

Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.

Introduction to Primary Cell Culture

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Primary Cell Culture Basics

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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