PC-9 (formerly known as PC-14)

90071810, human lung, Heterogeneous mixture of round cells and spindle shaped cells

• Synonym(s): PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells
• UNSPSC Code: 41106514

Properties

• product name: PC-9 (formerly known as PC-14), 90071810
• biological source: human lung
• growth mode: Adherent/suspension
• karyotype: Not specified
• morphology: Heterogeneous mixture of round cells and spindle shaped cells
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human adenocarcinoma

Cell Line Description

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

Application

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Seed cells at 2-4 x 10,000 cells/cm²; 5% CO₂; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

Other Notes

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