NUC101

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)

• Synonym(s): Rapid mammalian nuclei isolation kit
• UNSPSC Code: 12352207
• NACRES: NA.32

Properties

• usage: sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)
• Quality Level: 200
• shelf life: 1 yr at 2‑8 °C
• packaging: pkg of 1 kit
• application(s): cell analysis
• foreign activity: nuclease and protease, free
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Application

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Biochem/physiol Actions

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Other Notes

All procedures should be carried out on ice or 2-8°C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable