P7306
Polyethylene glycol/ dimethyl sulfoxide solution
Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma
Synonym(s):
PEG/DMSO Solution, Polyethylene Glycol Solution, Solution of PEG and DMSO
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About This Item
Recommended Products
grade
Hybri-Max™
Quality Level
sterility
0.2 μm filtered
product line
BioReagent
form
solution
mol wt
average mol wt 1,450
concentration
50 % (w/v)
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
SMILES string
C(CO)O
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key
LYCAIKOWRPUZTN-UHFFFAOYSA-N
Application
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.
Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.
Packaging
Packaged in sealed ampules under nitrogen.
Other Notes
Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.
Reconstitution
Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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