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S4762

Sigma-Aldrich

Streptavidin from Streptomyces avidinii

essentially salt-free, lyophilized powder, ≥13 units/mg protein

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

form

essentially salt-free, lyophilized powder

Quality Level

specific activity

≥13 units/mg protein

mol wt

~60 kDa

capacity

4 mol/mol (Biotin)

application(s)

research pathology

storage temp.

−20°C

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General description

Streptavidin derives its name from its bacterial source Streptomyces avidinii and from the hen egg-white protein, avidin, which has high affinity to biotin. Its homologous core shares 33% sequence similarity with avidin, as well as sharing a common tetrameric structure. It is a crystalline tetrameric protein, with a molecular weight of 4*15000Da. It binds four molecules of biotin. Streptavidin lacks carbohydrate and sulfur-containing amino acids.
Streptavidin is a crystalline protein isolated from the bacterium Streptomyces avidinii and possesses biotin-binding ability. It has four binding sites for biotin like avidin and binds four molecules of biotin. Streptavidin is now considered as a new type of biotin-binding protein.

Application

Streptavidin from Streptomyces avidinii has been used:
  • in wire functionalization
  • in streptavidin capture enzyme-linked immunosorbent assay (ELISA)
  • in microbubble preparation

Preparation Note

Affinity purified.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A rapid streptavidin-capture ELISA specific for the detection of antibodies to feline foamy virus.
Winkler IG et al
Journal of Immunological Methods, 207(1), 69-77 (1997)
Mapping of functional domains in the p22(phox) subunit of flavocytochrome b(559) participating in the assembly of the NADPH oxidase complex by "peptide walking".
Dahan I et al
The Journal of Biological Chemistry, 277(10), 8421-8432 (2002)
Timo Vögtle et al.
eLife, 8 (2019-08-23)
The immunoreceptor tyrosine-based inhibition motif (ITIM)-containing receptor G6b-B is critical for platelet production and activation. Loss of G6b-B results in severe macrothrombocytopenia, myelofibrosis and aberrant platelet function in mice and humans. Using a combination of immunohistochemistry, affinity chromatography and proteomics
Isolation and properties of streptavidin.
E A Bayer et al.
Methods in enzymology, 184, 80-89 (1990-01-01)
Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet N et al
Journal of Materials Chemistry, 14.17 , 2638-2642 (2004)

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