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59991-U

Supelco

Supel™ Carbon LC (2.7 µm) HPLC Columns

L × I.D. 5 cm × 3.0 mm, HPLC Column

Synonym(s):

Supel™ Carbon LC, 2.7 μm HPLC Column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Supel™ Carbon LC, 2.7 μm HPLC Column, column L × I.D. 5 cm × 3.0 mm

material

stainless steel column

Agency

suitable for USP L109

product line

Supel™

packaging

1 ea of

parameter

≤250 °C temp. limit
≤9000 psi max. pressure (620 bar)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

column L × I.D.

5 cm × 3.0 mm

surface area

155 m2/g

matrix

porous graphitic carbon (PGC) particle platform

particle size

2.7 μm

pore size

200 Å pore size

operating pH range

1-14

separation technique

mixed mode
reversed phase

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General description

Supel™ Carbon LC is a high-speed, ultra-high performance liquid chromatography column based on 200 Å porous graphitic carbon (PGC) particle technology. The PGC particle provides a 100% carbon plane for the analytes to interact with via hydrophobic interactions and through ionic interactions via the polar retention effect on graphite (PREG) effect. This particle design exhibits high column efficiency for polar compounds
and enhanced retention of compounds typically requiring hydrophilic interaction liquid chromatography (HILIC) to retain. Through the use of patent-pending synthetic procedures, a narrow particle size distribution is prevalent with this technology, further enhancing the chromatographic performance that this column can display.

Analysis Note

The target analytes include polar compounds, non-polar compounds, and structural isomers.

Footnote

Care & Use Guide for Supel™ Carbon LC, 2.7 μm HPLC Column

Recommended products

The use of a Supel™ Carbon LC 2.7 μm guard cartridge is recommended for the protection of your HPLC column. Guard cartridge kit, Cat. No. 59989-U, includes the appropriate Supel™ Carbon LC, 2.7 μm HPLC guard cartridge as well as the necessary holder. A pack of three replacement guard cartridges may be purchased using Cat. No. 59988-U. Additionally, a stand-alone guard cartridge holder may be purchased using Cat. No. 59999-U.

Legal Information

Supel is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
282194-04
282194-03
282194-02
282194-01
269819-04

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Evaluation of PGC Stationary Phases Under High-Temperature LC Conditions for the Analysis of Parabens in Food Samples
La Tella R, et al.
LC-GC, 36(5), 8-8 (2023)

Articles

UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC

Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.

Analysis: Activated Nucleotide Sugars by LC-MS/MS

LC-MS/MS method quantifies similar polar nucleotide activated sugars using Supel™ Carbon LC column for simultaneous analysis.

The Carbon Enigma: Material Fundamentals and Retention Properties for Porous Graphitic Carbon (PGC) Stationary Phases

A shortened review of fundamentals of Porous Graphitic Carbon (PGC) materials. What they are and how do they work as a stationary phase material in HPLC?.

Newly Developed Porous Graphitic Carbon in U/HPLC

Polar graphitic carbon enables UHPLC separation of highly polar pesticides, herbicides, and Vitamin D metabolites, advancing liquid chromatography capabilities.

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Supel™ Carbon LC U/HPLC Columns

Supel™ Carbon LC U/HPLC columns facilitate high-temperature and high-pressure separation of polar or charged compounds.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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