AB0300
Alkaline Phosphatase Blue Membrane Substrate Solution
alkaline phosphatase substrate, solution
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About This Item
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.83
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Product Name
Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate
form
solution
Quality Level
usage
sufficient for 400 mL working substrate
storage temp.
2-8°C
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General description
Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.
Application
Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
- to analyse direct alkaline phosphatase (AP) activity
- to perform alkaline phosphatase assays with colorimetric system
- to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3
Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.
Physical form
A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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