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Safety Information

198250

Sigma-Aldrich

Rose bengal

certified by the Biological Stain Commission, Dye content ≥80 %

Synonym(s):

4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal sodium salt

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About This Item

Empirical Formula (Hill Notation):
C20H2Cl4I4Na2O5
CAS Number:
632-69-9
Molecular Weight:
1017.64
Colour Index Number:
45440
Beilstein:
3645857
EC Number:
211-183-3
MDL number:
MFCD00005043
UNSPSC Code:
12171500
PubChem Substance ID:
24851870
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

100

form

powder

composition

Dye content, ≥80%

color

dark violet

solubility

H2O: 1 mg/mL

λmax

548 nm

ε (extinction coefficient)

≥90000 at 546-550 nm at 0.008 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]C(=O)c1c(Cl)c(Cl)c(Cl)c(Cl)c1C2=C3C=C(I)C(=O)C(I)=C3Oc4c(I)c([O-])c(I)cc24

InChI

1S/C20H4Cl4I4O5.2Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;/h1-2,29H,(H,31,32);;/q;2*+1/p-2

InChI key

UWBXIFCTIZXXLS-UHFFFAOYSA-L

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General description

Rose Bengal is the sodium salt of tetraiodotetrachlorouorescein, C20H2O5I4Cl4Na2. It is an anionic water-soluble xanthene dye.

Application

Rose bengal has several microbilogical staining applications including:
  • Conn′s technique for bacteria in soil and Kreyberg′s stain for keratin and mucus
  • counterstaining hematoxylin
  • staining cellular inclusions, together with Bismark brown



Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

198250-VAR:
198250-25G:4548173986302
198250-5G:4548173986319
198250-1KG:
198250-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS1232
0000253671
SHBQ2115
SHBP3528
SHBN9281

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