Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

803537

Sigma-Aldrich

Bis(NHS)PEG5

Synonym(s):

Bis-N-succinimidyl-(pentaethylene glycol) ester, Bis-succinimide ester-activated PEG compound

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C22H32N2O13
CAS Number:
756526-03-1
Molecular Weight:
532.50
UNSPSC Code:
12352200
NACRES:
NA.22

form

liquid

Quality Level

100

mol wt

532.5

reaction suitability

reagent type: cross-linking reagent

storage condition

desiccated

solubility

water: soluble

shipped in

ambient

storage temp.

−20°C

Related Categories

General description

By contrast to typical PEG reagents that contain heterogeneous mixtures of different PEG chain lengths, this PEG reagent is a homogeneous compound of defined molecular weight and spacer arm length, providing greater precision in optimization and characterization of crosslinking applications. Homobifunctional crosslinkers containing N-hydroxysuccinimide (NHS) esters are often used for low-resolution 3-D studies of protein structure and protein interaction analysis.

Features and Benefits

  • Reactive groups: NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • NHS esters react with—NH2 groups at pH 7-9, forming stable amide bonds
  • Water-soluble; compare to non-pegylated BS3 (also soluble) and DSS (water-insoluble)
  • Membrane-impermeable, allowing for cell surface labeling
  • Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
  • Polyethylene glycol spacer arms help maintain conjugate solubility
  • Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
  • PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
  • Ideal for small molecule or peptide conjugations

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

803537-100MG:

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
WI337107
UE288299
SB247715
QI225655

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

S Knoller et al.
The Journal of biological chemistry, 266(5), 2795-2804 (1991-02-15)
The superoxide (O2-) forming NADPH oxidase complex of resting phagocytes can be activated in a cell-free system by certain anionic amphiphiles, such as sodium dodecyl sulfate (SDS). For O2- production to occur, the participation of both membrane-associated and cytosol-derived components
G Mattson et al.
Molecular biology reports, 17(3), 167-183 (1993-04-01)
The various aspects of chemical crosslinking are addressed. Crosslinker reactivity, specificity, spacer arm length and solubility characteristics are detailed. Considerations for choosing one of these crosslinkers for a particular application are given as well as reaction conditions and practical tips
G W Cox et al.
Journal of immunology (Baltimore, Md. : 1950), 145(6), 1719-1726 (1990-09-15)
This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service