1.13303

Bactident^™ E. coli

suitable for detection

• Synonym(s): Test on E. Coli
• UNSPSC Code: 41116129
• NACRES: NA.84

Properties

• feature: ready-to-use
• Quality Level: 100
• manufacturer/tradename: Bactident^™
• technique(s): microbe id | specific enzyme detection: suitable
• input: sample(s) (environmental); water
• application(s): detection; environmental; food and beverages
• storage temp.: 2-8°C
• suitability: Citrobacter spp.; Escherichia coli; Proteus spp.

Description

General description

BACTIDENT^™ E. coli test strips are impregnated with 4-methylumbelliferyl-β-D-glucuronide (MUG), which is cleaved to form 4-methylumbelliferone in the presence of β-D-Glucuronidase. The light blue fluorescence upon excitation with long-wavelength UV light, indicates the presence of the enzyme. Tryptophanase activity is confirmed if the bacterial suspension turns red upon the addition of KOVACS’ reagent due to indole formation.

rapid identification kit for E. coli

Application

BACTIDENT^™ E. coli is used for the bioburden testing in drinking water. This test allows for the rapid detection of coliform organisms (E. coli) by testing the bacterial culture for the presence of enzymes β-D-Glucuronidase and Tryptophanase.

Linkage

Replaces: 1133030001

Analysis Note

Fluorescence
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Escherichia coli 194: +
- Escherichia coli ATCC 4351: +
- Proteus hauseri ATCC 13315: -
- Citrobacter freundii ATCC 8090: -
Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Escherichia coli 194: +
- Escherichia coli ATCC 4351: +
- Proteus hauseri ATCC 13315: +
- Citrobacter freundii ATCC 8090: -

Legal Information

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02,GHS05,GHS07,GHS02,GHS05,GHS07
• Signal Word: Danger-Danger
• Hazard Statements: H226 - H290 - H302 - H314 - H317 - H335 - H336
• Precautionary Statements: P210 - P280 - P210 - P233 - P280 - P302 + P352 - P305 + P351 + P338 - P313 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 96.8 °F
• Flash Point(C): 36 °C