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1.16493

Sigma-Aldrich

Urease

(from jack beans) solution in 50% glycerol, 1000 U/ml for biochemistry EC 3.5.1.5

Synonym(s):

Urease enzyme

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.21

form

liquid

pH

6.0-7.0 (20 °C in H2O)

density

1.10-1.30 g/cm3 at 20 °C

storage temp.

2-8°C

General description

Urease is a nickel-containing enzyme found in plants, bacteria, fungi, algae, and soil. It is a hexamer of a single chain of 840 amino acid residues. Urease is localized to mature seeds.

Biochem/physiol Actions

Ureases are metalloenzymes that catalyze the hydrolysis of urea to release ammonia (a nitrogen source for growth) and carbon dioxide. In plants, ureases are essential for the bioavailability/metabolism of nitrogen in nature and defense mechanisms. They exhibit internal peptides-mediated insecticidal action and fungicidal activity.

Analysis Note

Activity (Urea; pH 8.0; 25 °C): ≥ 1000 U/ml
Spec. activity (Urea; pH 8.0; 25 °C): about 500 

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

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Certificates of Analysis (COA)

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Articles

Analytical Method: Urea in Water and Wastewater, Enzymatic Determination

Urea in water and wastewater, enzymatic determination - Photometric determination using the indophenol blue method after cleavage of the urea

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