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Safety Information

203189

Sigma-Aldrich

Biotin-X-NHS, Water-Soluble

Synonym(s):

Biotin-X-NHS, Water-Soluble, Sulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate

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About This Item

Empirical Formula (Hill Notation):
C20H29N4O9S2 · Na
Molecular Weight:
556.59
UNSPSC Code:
41116133
NACRES:
NA.41

Assay

≥94% (HPLC)

Quality Level

100

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

off-white to tan

solubility

DMSO: 13 mg/mL
distilled water: 5.5 mg/mL

shipped in

ambient

storage temp.

2-8°C

General description

Readily couples biotin to primary amino groups of proteins under mild alkaline conditions. Ideally suited for labeling antibodies, hormones, or other polypeptide molecules containing lysine residues. Extended spacer arm provides maximum accessibility of biotin for avidin conjugates.
Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.

Other Notes

Gretch, D.R., et al. 1987. Anal. Biochem.163, 270.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

203189-50RMG:
203189-50MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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