292505

Diethylamine NONOate/AM

An esterase-sensitive and cell permeable, O²-acetoxymethylated diazeniumdiolate that acts as an intracellular nitric oxide (NO) donor (1.83 mol of NO/mol).

• Synonym(s): Diethylamine NONOate/AM
• Empirical Formula (Hill Notation): C₇H₁₅N₃O₄
• CAS Number: 213768-16-2
• Molecular Weight: 205.21
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (NMR)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: yellow
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A cell-permeable O²-acetoxymethylated diazeniumdiolate that donates nitric oxide (NO) following the action of intracellular esterases (1.83 mol of NO/mol). Shown to induce apoptosis and act as an antiproliferative agent in NO-sensitive human leukemia HL-60 (IC₅₀ = 8.3 µM) and U937 (IC₅₀ = 53 µM) cell lines. Also suppresses oxidized low-density lipoprotein-induced caspase-3 activation (~5 µM) in bovine aortic endothelial cells. Useful for temporal and spatial controlled NO availability.

An esterase-sensitive and cell permeable, O²-acetoxymethylated diazeniumdiolate that acts as an intracellular nitric oxide (NO) donor (1.83 mol of NO/mol). Shown to induce apoptosis and to act as an antiproliferative agent in NO-sensitive human leukemia HL-60 (IC₅₀ = 8.3 µM) and U937 (IC₅₀ = 53 µM) cell lines. Also suppresses oxidized low-density lipoprotein-induced caspase-3 activation in bovine aortic endothelial cells. Exhibits enhanced stability in physiological buffers. May also be useful for temporal and spatial controlled NO availability.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Intracellular nitric oxide (NO) donor

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 8.3 µM, 53 µM, acting as an antiproliferative agent in NO-sensitive human leukemia HL-60 and U937 cell lines, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 2 months at -20°C. Add to aqueous medium just prior to use.

Other Notes

Kotamraju, S., et al. 2001. J. Biol. Chem. 276, 17316.
Saavedra, J.E., et al. 2000. J. Med. Chem.43, 261.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable