346101-M

Androgen Receptor, His•Tag^®, Human, Recombinant, S. frugiperda

recombinant human, expressed in S. frugiperda

• Synonym(s): AR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant
• UNSPSC Code: 12352202

Properties

• biological source: human
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• storage temp.: −70°C
• Gene Information: human ... AR(367)

Description

General description

Research area: CELL CYCLE

Recombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag sequence and at the C-terminus to His•Tag sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. It is located at X chromosome and is expressed in various tissues. It consists of three primary functional domains: the N-terminal transcriptional regulation domain, the DNA binding domain (DBD), and the ligand binding domain.

Biochem/physiol Actions

The androgen receptor (AR) has significant biological effects on bone, muscle, prostate, adipose tissue, as well as on the reproductive, cardiovascular, immune, neural, and haemopoietic systems.

The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes.

Warning

Toxicity: Standard Handling (A)

Unit Definition

1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.

One unit is defined as 1 ng purified protein.

Physical form

In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Chen, G., et al. 2005. Prostate63, 395.
Estebanez-Perpina, E., et al. 2005, J. Biol. Chem.280, 8060.
Gao, S., et al. 2005. Mol. Endocrinol.19, 1792.
Hillmer, A.M., et al. 2005. Am. J. Hum. Genet.77, 140.
Kinoshita, M., et al. 2005, Int. J. Urol.12, 390.
Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A.102, 5156;
Marques, R.B., et al. 2005. Int. J. Cancer117, 221;
Metzger, E., et al. 2005. Nature437, 436;
Murthy, S., et al. 2005. Prostate64, 362;
Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA89, 5946.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1