Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

540220

Sigma-Aldrich

Purmorphamine

≥98% (HPLC), solid, Hedgehog signaling activator, Calbiochem

Synonym(s):

Purmorphamine

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C31H32N6O2
CAS Number:
483367-10-8
Molecular Weight:
520.62
UNSPSC Code:
12352116
NACRES:
NA.77

product name

Purmorphamine, A cell-permeable activator of Hedgehog signaling that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM).

Assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

off-white

solubility

DMSO: 200 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C31H32N6O2/c1-2-9-25(10-3-1)37-21-32-28-29(33-23-13-15-24(16-14-23)36-17-19-38-20-18-36)34-31(35-30(28)37)39-27-12-6-8-22-7-4-5-11-26(22)27/h4-8,11-16,21,25H,1-3,9-10,17-20H2,(H,33,34,35)

InChI key

FYBHCRQFSFYWPY-UHFFFAOYSA-N

General description

A cell-permeable 2,6,9-trisubstituted purine compound that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC50 = 1 µM) and lineage-committed preosteoblasts MC3T3-E1. Its effect can be synergized with that of BMP-4 (bone morphogenetic protein-4) and has been shown to induce transdifferentiation even in preadipocytes and myoblasts. Reported to induce osteogenesis by activation of the Hedgehog signaling pathway. A 50 mM (5 mg/192 µl) solution of Purmorphamine (Cat. No. 540223) in DMSO is also available.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
A cell-permeable activator of Hedgehog signaling that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM).
A cell-permeable purine compound that induces osteoblast differentiation in C3H10T1/2 multipotent mesenchymal progenitor cells (EC50 = 1 µM) and in MC3T3-E1 lineage-committed preosteoblasts. Also shown to induce transdifferentiation in pre-adipocytes and myoblasts. Its effects are reported to be synergistic with bone morphogenetic protein-4 (BMP-4). Reported to induce osteogenesis by activation of the Hedgehog signaling pathway.

Biochem/physiol Actions

Cell permeable: yes
EC50 = 1 µM for inducing osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2
Primary Target
Activation of the Hedgehog signaling pathway
Product does not compete with ATP.

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H341

Hazard Classifications

Muta. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

540220-5MG:
540220-VMG:
540220-20MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Hynek Wichterle et al.
Methods in molecular biology (Clifton, N.J.), 482, 171-183 (2008-12-18)
A growing number of specific cell types have been successfully derived from embryonic stem cells (ES cells), including a variety of neural cells. In vitro generated cells need to be extensively characterized to establish functional equivalency with their in vivo
Hynek Wichterle et al.
Current protocols in stem cell biology, Chapter 1, Unit 1H-Unit 1H (2008-09-05)
Controlled differentiation of embryonic stem (ES) cells into clinically relevant cell types is a fundamental goal of stem cell research. This unit describes one of the most efficient protocols for conversion of mouse ES cells into a defined type of
Lisa Schwarz et al.
Cells, 11(8) (2022-04-24)
Miro1 has emerged as an interesting target to study Parkinson's disease-relevant pathways since it is a target of PINK1 and Parkin. Miro1 is a mitochondrial GTPase with the primary function of facilitating mitochondrial movement, and its knockout in mice is
Max Meuser et al.
Development (Cambridge, England), 149(1) (2022-01-13)
The patterned array of basal, intermediate and superficial cells in the urothelium of the mature ureter arises from uncommitted epithelial progenitors of the distal ureteric bud. Urothelial development requires signaling input from surrounding mesenchymal cells, which, in turn, depend on
Asuka Morizane et al.
Methods in molecular biology (Clifton, N.J.), 1018, 11-19 (2013-05-18)
Pluripotent stem cells are promising potential sources for cell replacement therapy and are useful research tools for exploring disease mechanisms. Neural cells are one of the cell types that have been most efficiently differentiated through several established protocols. This chapter
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service