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Safety Information

70526

Sigma-Aldrich

Clonables Ligation/Transformation Kit - Novagen

Simple, reproducible ligation and transformation—in as little as 23 minutes

Synonym(s):

Clonables Kit

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About This Item

UNSPSC Code:
41106617
NACRES:
NA.54

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

dry ice

storage temp.

−70°C

General description

The Clonables Kit enables convenient, dependable, high-efficiency ligation and transformation of any compatible DNA ends. The kit features a unique, universal Ligation Premix, containing ligase, buffer, and cofactors, which supports ligation of any type of DNA cohesive or blunt ends in a 15-minute reaction. Ligated DNA is transformed into NovaBlue Singles Competent Cells, which use a streamlined protocol that takes less than 8 minutes for ampicillin-resistant plasmids and 38 minutes for other antibiotic-resistant plasmids. This kit can be used with a variety of cloning vectors and is compatible with any type of DNA end, without altering the ends or desired cloning junctions. The Clonables Kit contains sufficient reagents to perform 11 ligation and transformation reactions, and includes a control vector and insert mix to verify performance.

Features and Benefits

  • Fast! 15-minute ligation, 8-minute transformation with ampicillin selection
  • Ligation components premixed to decrease pipetting steps and increase reliability
  • One reaction, optimized for cohesive ends, single base overhangs, and blunt ends
  • Single-use competent cells eliminate need to aliquot, freeze/thaw, or waste partially used vials
  • Compatible with PCR buffer, TE, restriction enzyme buffer, or End Conversion Mix

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

70526-3:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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