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Safety Information

71753-M

Sigma-Aldrich

LB Broth, Miller - Novagen

Novagen®, granular, pack of 500 g or 5 kg, for Escherichia coli

Synonym(s):

Luria-Bertani broth, Lysogeny broth

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.54

form

granular

Quality Level

100

manufacturer/tradename

Novagen®

packaging

pack of 500 g or 5 kg

storage condition

OK to freeze

technique(s)

microbiological culture: suitable

application(s)

microbiology

storage temp.

10-30°C

suitability

Escherichia coli

General description

LB Broth MILLER is a granulated medium for the cultivation of E. coli on scales ranging from small cultures to fermentation. The granules ensure rapid and uniform dissolution in water, and prevent clumping of the medium and inhalation of the airborne powder. It is supplied in several formats.

Application

LB Broth, Miller - Novagen has been used as a culture medium for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa strains.

Components

LB Broth, Miller contains yeast extract(5g), peptone from casein (10 g), and sodium chloride(10 g)

Warning

Toxicity: Standard Handling (A)

Preparation Note

EasyPak is an aluminum foil pouch containing 25 g granulated medium sufficient for 1 L culture. Just add the EasyPak contents to 1 L water, microwave for 2 min to sterilize*, and it is ready to use.

*Microwaving is sufficient if an antibiotic will be added to the prepared media.

Legal Information

71753-5, 71753-6 and 71753-7 are not available for sale in Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, and UK.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

71753-5:
71753-1EA:
71753-4:
71753-6:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

L7275

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Crystal violet-based assay is widely used to evaluate the early stages of bacterial biofilm formation. Here, we modified it to study biofilm formation in medical tubing. While cost-effective and requiring no sophisticated equipment, our method provided quantitative and qualitative distinctions

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