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Safety Information

HTS076RTA

Sigma-Aldrich

Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells

Human

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.84
biological source:
human
manufacturer/tradename:
Ready-to-Assay

Product Name

Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells, Human PAF GPCR frozen cells for Calcium Flux FLIPR Assays.

biological source

human

Quality Level

100

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

NCBI accession no.

NM_000952

detection method

fluorometric

shipped in

dry ice

General description

Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Platelet-activating factor (PAF) is a bioactive phospholipid that was originally characterized by its ability to induce platelet aggregation, but was subsequently shown to mediate angiogenesis, inflammation and neural development. The PAF receptor is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and cytokine and leukotriene release (Stafforini et al., 2003). Millipore’s cloned human PAF-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PAF expression on the cell surface and contains high levels of the promiscuous G protein G15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PAF and its ligands.

Cell Line Description

Host cells: Chem-1

Biochem/physiol Actions

Protein Target: PAF
Target Sub-Family: Platelet Activating Factor

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HTS076RTA:

Certificates of Analysis (COA)

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