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Safety Information

HTS095RTA

Sigma-Aldrich

Ready-to-Assay Kappa Opioid Receptor Frozen Cells

Human

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.84

product name

Ready-to-Assay Kappa Opioid Receptor Frozen Cells,

biological source

human

Quality Level

100

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

NCBI accession no.

NM_000912

detection method

fluorometric

shipped in

dry ice

General description

EMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Opiates derived from the opium poppy, Papaver somniferum, have been used for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, μ, κ, and d, that activate Gi/o to reduce intracellular cAMP levels. Most clinically used opioids function by activation of the μ opioid receptor. Activation of the κ opioid receptor by selective agonists also produces analgesia, primarily mediated by spinal sites, but causes dysphoria and psychosis instead of euphoria. The κ receptor at central and peripheral sites is also largely responsible for the anti-diarrheal effects of opiates (Dhawan et al., 1996). EMD Millipore’s cloned human κ-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant κ expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.

Cell Line Description

GPCR Cell Lines
Host cells: Chem-1

Biochem/physiol Actions

GPCR Class: A
Protein Target: Kappa / OP2 / KOP / KOR
Target Sub-Family: Opioid

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.

Fifty (50) mL of Media Component.

Other Notes

Limited to a single use and shall not be propagated and/or re-frozen by licensee.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HTS095RTA:

Certificates of Analysis (COA)

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