UFC5003
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 3 kDa
Synonym(s):
Centrifugal concentrator, 3 kDa MWCO, 0.5 mL sample volume
About This Item
Recommended Products
material
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Ultra
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
pore size
3 kDa MWCO
shipped in
ambient
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
Application
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Physical form
Legal Information
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Protocols
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
Related Content
Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.
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