199664

Sudan Black B

certified by the Biological Stain Commission

• Synonym(s): Ceres black BN, Fat Black HB, Solvent Black 3
• Empirical Formula (Hill Notation): C₂₉H₂₄N₆
• CAS Number: 4197-25-5
• Molecular Weight: 456.54
• Colour Index Number: 26150
• Beilstein: 723248
• EC Number: 224-087-1
• MDL number: MFCD00006919
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851932
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• technique(s): microbe id | staining: suitable
• color: dark brown to very dark brown
• mp: 120-124 °C (lit.)
• λmax: 415 nm (2nd); 598 nm
• ε (extinction coefficient): ≥20000 at 596-603 nm in ethanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
• InChI: 1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
• InChI key: YCUVUDODLRLVIC-VPHDGDOJSA-N

Description

Application

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Biochem/physiol Actions

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Suitability

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H341
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves