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75482

Sigma-Aldrich

Gram′s decolorizer solution

for Gram staining

Synonym(s):

Acetone ethanol mixture

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About This Item

CAS Number:
37348-17-7
MDL number:
MFCD00240361
UNSPSC Code:
12171500
NACRES:
MA.02

Quality Level

100

form

liquid

technique(s)

microbe id | staining: suitable

refractive index

n20/D 1.361

density

0.80 g/mL at 20 °C

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

General description

Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolorizer solution is a mixture of ethanol and acetone.

Application

Gram′s decolorizer solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolorizer dissolves the lipid layer from the gram-negative cells.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319,H336

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Supplementary Hazards

EUH066

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

68.0 °F

Flash Point(C)

20 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Type 1 petroleums
Hazardous rank II
Water insoluble liquid

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

75482-BULK-F:
75482-VAR-F:
75482-250ML-F:4548173288536
75482-2.5L-F:4548173288529

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM4550
BCCL6927
BCCJ9420
BCCK8710
BCCJ3406

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