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C2734

Sigma-Aldrich

5-Carboxytetramethylrhodamine

≥95% purity (HPLC), solid

Synonym(s):

5-TAMRA

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About This Item

Empirical Formula (Hill Notation):
C25H22N2O5
CAS Number:
91809-66-4
Molecular Weight:
430.45
MDL number:
MFCD01073638
UNSPSC Code:
12171500
NACRES:
NA.47

product name

5-Carboxytetramethylrhodamine,

Assay

≥95% (HPLC)

Quality Level

100

form

solid

solubility

water: slightly soluble

ε (extinction coefficient)

≥85000-97000 at 539-545 nm in methanol

fluorescence

λex ~543 nm; λem ~570 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChI key

YMZMTOFQCVHHFB-UHFFFAOYSA-N

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General description

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Application

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Biochem/physiol Actions

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C2734-10MG-PW:
C2734-10MG:
C2734-VAR:
C2734-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
MKCT6671
MKCR5570
MKCQ1970
MKCM5246
MKCH9058

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