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10831

Sigma-Aldrich

Histopaque®-1083

sterile-filtered, density: 1.083

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

400

form

liquid

shelf life

Expiry date on the label.

impurities

endotoxin, tested

density

1.083

application(s)

hematology
histology

storage temp.

2-8°C

Related Categories

General description

Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Application

Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Features and Benefits

Histopaque®-1083 offers numerous advantages, such as:

  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principle

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

10831-PH:
10831-100ML:4548173373454
10831-PROC:
10831PROC:
10831-VAR:
10831-6X100ML:4548173373461
10831-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL4897
RNBL5440
RNBK9776
RNBK6510
RNBK4686

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Gangduo Wang et al.
Toxicology and applied pharmacology, 237(2), 188-195 (2009-04-01)
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The Journal of thoracic and cardiovascular surgery, 159(5), 1825-1835 (2019-07-30)
Extracellular vesicles (EVs) are promising therapeutics for cardiovascular disease, but poorly-timed delivery might hinder efficacy. We characterized the time-dependent response to endothelial progenitor cell (EPC)-EVs within an injectable shear-thinning hydrogel (STG+EV) post-myocardial infarction (MI) to identify when an optimal response
Bella S Guerrouahen et al.
Pediatric blood & cancer, 53(6), 1132-1135 (2009-06-02)
Tyrosine kinase inhibitors, such as imatinib, have dramatically improved the outcomes for patients with selected cancers. For imatinib, western blotting of phospho-CrkL was an insensitive, indirect, and descriptive method to determine drug efficacy. Greater use of targeted therapies should involve
Ahmad J H Albaghdadi et al.
Scientific reports, 9(1), 6528-6528 (2019-04-27)
Polycystic ovary syndrome (PCOS) is characterized by failure of ovulation and is associated with obesity and chronic inflammation. Recent evidence suggests that anomalous activation of ovarian macrophages and numerical and functional deficits in the Th17 (CD4+IL17A+) and the CD4+CD25+CD127low Tregs
Qiyue Hu et al.
Scientific reports, 8(1), 7675-7675 (2018-05-18)
Interleukin-15 (IL-15) can promote both innate and adaptive immune reactions by stimulating CD8+/CD4+ T cells and natural killer cells (NK) while showing no effect in activating T-regulatory (Treg) cells or inducing activation-associated death among effector T cells and NK cells.
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