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Safety Information

56822

Sigma-Aldrich

Immersion oil

for microscopy

Synonym(s):

immersion medium for light microscopy

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About This Item

MDL number:
MFCD00131369
UNSPSC Code:
12171500
NACRES:
NA.47

biological source

synthetic

Quality Level

100

grade

for microscopy

form

liquid

refractive index

n20/D 1.516

viscosity

100-120 mPa.s(20 °C)

density

1.025 g/mL at 20 °C

application(s)

hematology
histology

storage temp.

room temp

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General description

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power.

Application

Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.

Principle

Immersion oil is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H410

Precautionary Statements

P273 - P391 - P501

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PRTR

Class I Designated Chemical Substances

FSL

Group 4: Flammable liquids
Type 3 petroleums
Hazardous rank III
Water insoluble liquid

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

56822-6X250ML:
56822-BULK:
56822-1L:
56822-VAR:
56822-250ML:
56822-50ML:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM8231
BCCM2069
BCCL6601
BCCL9234
BCCL6600

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