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Safety Information

62314

Sigma-Aldrich

Lipase Substrate

for the titrimetric determination of enzyme activity

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32

grade

for the titrimetric determination of enzyme activity

Quality Level

100

form

liquid

technique(s)

titration: suitable

refractive index

n20/D 1.36

density

1.05 g/mL at 20 °C

storage temp.

2-8°C

Physical form

aqueous solution with 4.5 mM triolein; 1 M NaCl; 13% (w/v) Triton™ X-100

Other Notes

Assay of microbial lipases with emulsified trioleoyl glycerol; the fatty acids released are titrated with a pH stat

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

62314-100ML-F:
62314-BULK-F:
62314-VAR-F:
62314-500ML-F:

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Certificates of Analysis (COA)

Lot/Batch Number
BCBW5203
BCBR3902V
1445066V
BCBD6629V
1445066

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A new assay of microbial lipases with emulsified trioleoyl glycerol.
N Peled et al.
Analytical biochemistry, 112(2), 219-222 (1981-04-01)
Hannes Schmidinger et al.
Chembiochem : a European journal of chemical biology, 7(3), 527-534 (2006-02-14)
Protein and small-molecule microarrays are useful tools for high-throughput analysis of DNA-protein, protein-protein, and protein-small molecule interactions. Here we report on novel microarrays for activity screening of lipases and esterases based on phosphonic acid ester inhibitors. These compounds are activity
H Schmidinger et al.
Amino acids, 30(4), 333-350 (2006-06-15)
In the postgenomic era new technologies are emerging for global analysis of protein function. The introduction of active site-directed chemical probes for enzymatic activity profiling in complex mixtures, known as activity-based proteomics has greatly accelerated functional annotation of proteins. Here
Hannes Schmidinger et al.
Chembiochem : a European journal of chemical biology, 6(10), 1776-1781 (2005-08-12)
Lipases and esterases are responsible for carboxylester hydrolysis inside and outside cells and are useful biocatalysts for (stereo)selective modification of synthetic substrates. Here we describe novel fluorescent suicide inhibitors that differ in structure and polarity for screening and discrimination of
G Hofer et al.
Free radical research, 23(4), 317-327 (1995-10-01)
We report on a new method for the determination of lipid oxidation in lipoproteins and plasma. The biological lipid system is preloaded with a fluorescent analog of phosphatidylcholine containing diphenylhexatriene (DPH) propionic acid covalently linked to the sn-2 position. When
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