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75641

Sigma-Aldrich

Poly(2′-deoxyguanyl-2′-deoxycytidylic acid) sodium salt

ampule of >10 A260

Synonym(s):

Poly(dG-dC) sodium salt

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352106
NACRES:
NA.52

grade

for molecular biology

Quality Level

form

liquid

packaging

ampule of >10 A260

solubility

H2O: complete 1 mL/vial, clear, colorless

storage temp.

−20°C

SMILES string

[P](=O)(O[C@@H]3[C@H](O[C@H](C3)[n]4c5nc(nc(c5nc4)O)N)CO[P](=O)(O)O)(OC[C@H]1O[C@H](C[C@@H]1O)N2C=CC(=NC2=O)N)O

InChI

1S/C19H26N8O13P2/c20-12-1-2-26(19(30)23-12)13-3-8(28)10(38-13)5-37-42(34,35)40-9-4-14(39-11(9)6-36-41(31,32)33)27-7-22-15-16(27)24-18(21)25-17(15)29/h1-2,7-11,13-14,28H,3-6H2,(H,34,35)(H2,20,23,30)(H2,31,32,33)(H3,21,24,25,29)/t8-,9-,10+,11+,13+,14+/m0/s1

InChI key

IBZPKQZTZXRCKY-WKSZEZMPSA-N

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Application

Poly (2′-deoxyguanyl-2′-deoxycytidylic acid) sodium salt was used as standard in gel electrophoresis to analyze the products of Klenow exo fragment of polymerase I.

Biochem/physiol Actions

Poly (2′-deoxyguanyl-2′-deoxycytidylic acid) is a protonated polynucleotide structure frequently used to study the conformational changes of DNA molecules.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Alexander B Kotlyar et al.
Nucleic acids research, 33(2), 525-535 (2005-01-28)
In this paper, we describe a production procedure of the one-to-one double helical complex of poly(dG)-poly(dC), characterized by a well-defined length (up to 10 kb) and narrow size distribution of molecules. Direct evidence of strands slippage during poly(dG)-poly(dC) synthesis by
T Kohwi-Shigematsu et al.
Cell, 43(1), 199-206 (1985-11-01)
Supercoiled plasmid DNAs (at bacterial superhelical density) harboring the homopurine-homopyrimidine sequence, poly(dG)-poly(dC), were reacted with bromoacetaldehyde (BAA), a reagent that reacts with unpaired DNA bases. Not only did the poly(dG)-poly(dC) sequence react with BAA but, surprisingly, neighboring sequences located 3'

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