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Safety Information

A7345

Millipore

Anti-V5 Agarose Affinity Gel antibody produced in mouse

purified immunoglobulin, clone V5-10

Synonym(s):

Monoclonal Anti-V5

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About This Item

MDL number:
MFCD03457579
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

Quality Level

200

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V5-10, monoclonal

analyte chemical class(es)

proteins (V5)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

capacity

2.5 nmol/mL, gel (V5 fusion protein)

shipped in

wet ice

storage temp.

2-8°C

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General description

Anti-V5 Agarose Affinity Gel is made of purified immunoglobulin fraction of monoclonal anti-V5 isolated from ascites fluid of the V5-10 hybridoma coupled to cyanogen bromide-activated beaded agarose. The purified monoclonal antibody is immobilized on agarose.
The Anti-V5 Agarose Affinity Gel is composed of the monolonal anti-V5 antibody conjugated to cyanogen bromide-activated beaded agarose. The antibody recognizes the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Anti-V5 Agarose Affinity Gel antibody produced in mouse has been used in immunoprecipitation and recombinant protein purification.

Biochem/physiol Actions

Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide ′affinity handles′ designed to enable the selective identification and purification of the protein of interest. The addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the bioactivity of the protein, or with the biodistribution of the tagged product. Many recombinant proteins have been engineered to express a short sequence derived from the V5 from simian virus known as V5-tag. Anti-“tag” agarose conjugates may facilitate the detection, isolation and purification of these proteins.

Physical form

Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline, containing 15 mM sodium azide.

Storage and Stability

For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A7345-VAR:
A7345-1ML:
A7345-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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