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Safety Information

A9231

Sigma-Aldrich

Acridine Orange solution

2% in H2O

Synonym(s):

3,6-Acridinediamine, N,N,N′,N′-tetramethyl-, monohydrochloride, 3,6-Bis(dimethylamino)acridine hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C17H19N3 · HCl
CAS Number:
65-61-2
Molecular Weight:
301.81
MDL number:
MFCD00005029
UNSPSC Code:
12171500
PubChem Substance ID:
329771043
NACRES:
NA.47

form

liquid

Quality Level

100

shelf life

18 mo.

concentration

2% in H2O

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

N(C)(C)c1cc2nc3c(cc2cc1)ccc(c3)N(C)C

InChI

1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

InChI key

DPKHZNPWBDQZCN-UHFFFAOYSA-N

Related Categories

General description

Acridine Orange (C.l. 46005) is an acridine dye with a ring structure and overall small planar conjugated system.

Application

Acridine orange is a DNA intercalating dye that is a recommended DNA stain for flow cytometry. It differentially stains double-stranded and single-stranded nucleic acids.

It also has the following applications-
  • staining of RNA and acid mucins
  • detecting apoptosis
  • staining blood smears to diagnose malaria
  • identifying and enumerating granulocytes in fish
  • staining eukaryotic cells to demonstrate low pH structures

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A9231-VAR:
A9231-10ML:
A9231-BULK:
A9231-10ML-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW2945
MKCV6647
MKCW8516
MKCT7107
MKCS9258

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Matthew P Harris et al.
PLoS genetics, 4(10), e1000206-e1000206 (2008-10-04)
The genetic basis of the development and variation of adult form of vertebrates is not well understood. To address this problem, we performed a mutant screen to identify genes essential for the formation of adult skeletal structures of the zebrafish.
Hong-xiang Wang et al.
Zhonghua yi xue za zhi, 92(26), 1828-1831 (2012-09-05)
To ascertain the epididymal sperm nuclear maturity in obstructive azoospermia (OA) patients. A total of 81 infertile males from andriatry clinic of Renji hospital were selected, including 32 OA patients (OA group) and 49 asthenospermia patients (asthenospermia group). Another 32
Grace Emily Okuthe
Acta histochemica, 115(2), 178-184 (2012-07-17)
Oogenesis involves a sequence of cellular divisions and developmental changes leading to the formation of oocytes, whose role in development is to transfer genomic information to the next generation. During this process, the gene expression pattern changes considerably concomitant with
Marion Herbette et al.
Cells, 9(9) (2020-09-12)
Deposition of histone H3 lysine 4 (H3K4) methylation at promoters is catalyzed by the SET1/COMPASS complex and is associated with context-dependent effects on gene expression and local changes in chromatin organization. The role of SET1/COMPASS in shaping chromosome architecture has
Donald P Evenson
Methods in molecular biology (Clifton, N.J.), 927, 147-164 (2012-09-21)
The SCSA(®) is the pioneering assay for the detection of damaged sperm DNA and altered proteins in sperm nuclei via flow cytometry of acridine orange (AO) stained sperm. The SCSA(®) is considered to be the most precise and repeatable test
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