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Safety Information

A9275

Sigma-Aldrich

Avidin from egg white

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

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About This Item

CAS Number:
1405-69-2
EC Number:
215-783-6
MDL number:
MFCD00130572
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

egg white

Quality Level

200

product line

BioUltra

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

specific activity

≥10 units/mg protein (E1%/280)

mol wt

glycoprotein 66 kDa
subunit 16 kDa

storage temp.

2-8°C

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General description

Avidin protein is a homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies. The product has also been used as a blocking agent in ELISA applications.

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Preparation Note

Affinity purified

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A9275-EW:
A9275-100MG:
A9275-5MG:
A9275-25MG:
A9275-1MG:
A9275-VAR:
A9275-10MG:
A9275-2MG:
A9275-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000412856
0000412857
0000412857
0000412856
0000398242

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Q Wang et al.
The American journal of physiology, 275(5 Pt 2), R1584-R1592 (1998-10-29)
We examined the effect of endotoxemia in mice on protein and mRNA levels for the acute phase proteins complement C3 and serum amyloid A (SAA) in jejunal mucosa. Endotoxemia was induced in mice by the subcutaneous injection of 250 microg
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Efficient gene transfer to the vascular wall via intravenous vector injection would be useful for experimental vascular biology and gene therapy. Initial studies of lentiviral vector tropism suggested that intravenously injected vectors do not transduce murine vascular tissue; however, there
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