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Safety Information

B2025

Sigma-Aldrich

Brilliant Blue G - Colloidal Concentrate

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

form

solution

Quality Level

200

storage temp.

room temp

General description

Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.

Application

Brilliant Blue G - Colloidal Concentrate has been used in the staining
  • protein fragments post in vitro pepsin digestion
  • ovarian tumor fluid proteome prior to profiling
  • the transcription factor (TFIID) complex from Saccharomyces cerevisiae

Reconstitution

The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

B2025-1EA:4548174007365
B2025-VAR:
B2025-BULK:
B2025PROC:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ0016
SLCJ6088
SLCC7506
SLBT3592
SLBQ0154V

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Proteomic analysis of ovarian cancer tumor fluid is a rich source of potential biomarkers
Poersch A, et al.
Journal of Proteomics and Bioinformatics, 5(2), 2-2 (2014)
Elena Barbieri et al.
Journal of aging research, 2011, 845379-845379 (2011-06-02)
This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional and biochemical analyses. Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mitochondrial mass and membrane potential were
Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis
Candiano G, et al.
Electrophoresis, 25(9), 1327-1333 (2004)
A proteomic signature of ovarian cancer tumor fluid identified by highthroughput and verified by targeted proteomics
Poersch A, et al.
Journal of proteomics, 145(2), 226-236 (2016)
Antonis Ververis et al.
Cellular and molecular life sciences : CMLS, 73(1), 163-184 (2015-07-15)
Katanins are microtubule (MT)-severing AAA proteins with high phylogenetic conservation throughout the eukaryotes. They have been functionally implicated in processes requiring MT remodeling, such as spindle assembly in mitosis and meiosis, assembly/disassembly of flagella and cilia and neuronal morphogenesis. Here
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Protocols

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.

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