Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

C3228

Sigma-Aldrich

CelLytic™ MT Cell Lysis Reagent

For mammalian tissues

Synonym(s):

Cell lysis buffer, Cell lysis reagent

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41116134
NACRES:
NA.56

Application

CelLytic™ MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).

Features and Benefits

  • Gentle: Non-denaturing and does not interfere with downstream applications
  • Convenient: Provided ready to use

Other Notes

CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.

Quality

It has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C3228-500ML:
C3228-VAR:
C3228-50ML:
C3228-500ML-PW:
C3228-BULK:
C3228-50ML-PW:

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000422504
0000422503
0000422503
0000389705
0000389705

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mechanistic Target of Rapamycin Complex 1 Promotes the Expression of Genes Encoding Electron Transport Chain Proteins and Stimulates Oxidative Phosphorylation in Primary Human Trophoblast Cells by Regulating Mitochondrial Biogenesis
Rosario FJ, et al.
Scientific Reports, 9(1), 246-246 (2019)
Leticia E Camacho et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(2), R101-R109 (2017-05-12)
Intrauterine growth restriction (IUGR) is associated with persistent metabolic complications, but information is limited for IUGR infants. We determined glucose-stimulated insulin secretion (GSIS) and insulin sensitivity in young lambs with placental insufficiency-induced IUGR. Lambs with hyperthermia-induced IUGR (
Joseph S Marino et al.
The Journal of biological chemistry, 291(50), 25776-25788 (2016-10-28)
Glucocorticoids (GCs) regulate energy supply in response to stress by increasing hepatic gluconeogenesis during fasting. Long-term GC treatment induces hepatic steatosis and weight gain. GC signaling is coordinated via the GC receptor (GR) GRα, as the GRβ isoform lacks a
Pasireotide protects mammalian cochlear hair cells from gentamicin ototoxicity by activating the PI3KAkt pathway
Kucharava K, et al.
Cell death & disease, 10(2), 110-110 (2019)
Aerobic exercise, but not metformin, prevents reduction of muscular performance by AMPk activation in mice on doxorubicin chemotherapy
de Lima EA, et al.
Journal of Cellular Physiology, 233(12), 96529662-96529662 (2018)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service