CGD1

Cell Growth Determination Kit, MTT based

• Synonym(s): Mitochondrial activity assay
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• packaging: pkg of 1 kit
• Quality Level: 200
• technique(s): UV/Vis spectroscopy: suitable; protein quantification: suitable
• Amax: 570 nm
• application(s): cell analysis; detection
• detection method: colorimetric
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Application

Cell Growth Determination Kit, MTT based has been used:

• to measure the cell viability of preosteoblastic cells
• in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
• to determine the cell viability of primary cervical epithelial cells

Packaging

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

Safety Information

• Pictograms: GHS02,GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H319,H336,H341
• Precautionary Statements: P202 - P210 - P233 - P240 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 3 - Flammable liquids
• Flash Point(F): 53.6 °F - closed cup
• Flash Point(C): 12 °C - closed cup