CGP1

Glutathione Peroxidase Cellular Activity Assay Kit

Sufficient for 100 colorimetric tests

• Synonym(s): Cellular glutathione peroxidase, c-GPx
• UNSPSC Code: 12161503
• NACRES: NA.32

Properties

• Quality Level: 200
• usage: sufficient for 100 tests
• shelf life: Kit is stable for 24 months when unopened.
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Suitability

Suitable for the measureent of glutathione peroxidase in tissue extracts

Principle

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Other Notes

This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.

Kit Components Only

• tert-Butyl hydroperoxide 1 mL

Safety Information

• Pictograms: GHS02,GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H226,H242,H302,H311,H314,H317,H330,H335,H341,H411
• Precautionary Statements: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P370 + P378
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 5.2 - Organic peroxides and self-reacting hazardous materials