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CRISPR11V

Sigma-Aldrich

CRISPR-Lenti Human EMX1 Positive Control Transduction Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

form

liquid

Quality Level

200

packaging

vial of 200 μL

concentration

1x106  VP/ml (via p24 assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

General description

The Sigma CRISPR-LENTI HUMAN EMX1 POSITIVE CONTROL TRANSDUCTION PARTICLES are a critical positive control to monitor transduction efficiency for lenti CRISPRs. This control is produced from the sequence-verified CRISPR lentiviral plasmid targeting human EMX1. EMX1 is one of the members of the EMX homeobox family, which are expressed in the developing cerebrum and involved in specification of positional identity and neural cell proliferation. This targets a validated EMX1 CRISPR site, which serves as an experimental control for Sigma lenti CRISPRs. The Sigma lenti CRISPR positive control is a one-component system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA plasmid targeting the human EMX1 gene, with both Puromycin and GFP co-expressed with Cas9.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.

Application

Functional Genomics/Screening/Target Validation
  • Positive control, CRISPR lentivirus, viral format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crisprs.

Features and Benefits

Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPRs (virus format). Allows for validation of your system with the lenti CRISPR/Cas9 system. A positive result in a mis-match detection assay will indicate validation of your system.

Physical form

200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

Legal Information

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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