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Key Documents

Safety Information

D8037

Sigma-Aldrich

Driselase™ Basidiomycetes sp.

BioReagent, suitable for plant cell culture

Synonym(s):

Driselase™ from Basidiomycetes sp.

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About This Item

CAS Number:
85186-71-6
EC Number:
286-055-3
MDL number:
MFCD00130978
UNSPSC Code:
10171502
NACRES:
NA.72

product line

BioReagent

Quality Level

200

form

powder

composition

Protein, ≥10% biuret

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

−20°C

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Application

Driselase™ Basidiomycetes sp. has been used:

  • in spheroplast preparation from Coccomyxa cells
  • in a CRISPR/Cas9-based mutagenesis protocol for Brachypodium distachyon and its allopolyploid relative, Brachypodium hybridum
  • for cell wall digestion to perform whole-mount immunolocalization of Lotus japonicus root tissue

Biochem/physiol Actions

Driselase™ is a natural mixture of enzyme activities (fungal carbohydrates) used to digest plant cell walls to facilitate the maceration of plant materials, protoplast formation, and extraction processes. Driselase releases cell wall carbohydrates. This formulation contains enzyme activities of cellulose, endo-1,3-β-glucanase, and xylanase.

Other Notes

Crude powder containing laminarinase, xylanase and cellulase.

Legal Information

Driselase is a trademark of ASKA Animal Health Co. Ltd.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D8037-5G:
D8037-VAR:
D8037-BULK:
D8037-1G:
D8037-25G:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dieter Hackenberg et al.
Plant physiology, 172(2), 1154-1166 (2016-08-24)
In this study, we report the functional characterization of heterotrimeric G-proteins from a nonvascular plant, the moss Physcomitrella patens. In plants, G-proteins have been characterized from only a few angiosperms to date, where their involvement has been shown during regulation
M C Ralet et al.
Carbohydrate research, 263(2), 227-241 (1994-10-17)
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes
D P Blowers et al.
Plant physiology, 86(2), 505-509 (1988-02-01)
Plasma membrane vesicles from wild carrot cells grown in suspension culture were isolated by aqueous two-phase partitioning, and ATP-dependent phosphorylation was measured with [gamma-(32)P]ATP in the presence and absence of calcium. Treatment of the carrot cells with the cell wall
T Ishii et al.
Carbohydrate research, 248, 179-190 (1993-10-04)
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose
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