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Safety Information

F3013

Sigma-Aldrich

Ferredoxin from Spinacia oleracea (spinach)

lyophilized powder, Ferredoxin ≥15 wt. %

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About This Item

CAS Number:
9040-09-9
MDL number:
MFCD00131049
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

spinach (Spinacia oleracea)

Quality Level

200

form

lyophilized powder

composition

Ferredoxin, ≥15 wt. %

solubility

water: 2 mg/mL, clear, faintly brown

application(s)

cell analysis

storage temp.

−20°C

Gene Information

spinach ... SOVF(137530)

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General description

Ferredoxin I (Fd I) from Spinacia oleracea comprises 97 amino acid and is an iron sulfur cluster (2Fe-2S). It is synthesized in the chloroplast. The structure of Fd I has conserved active site.

Application

Ferredoxin from Spinacia oleracea (spinach) has been used:
  • as a substrate for spinach ferredoxin reductase,
  • for reconstitution with chlorophyll-A for scanning electron microscope (SEM) dispersive X-ray analyzer (EDX) integrated analysis,
  • as a reductant in adenylylsulfate (APS) reductase activity of P. aeruginosa

Biochem/physiol Actions

Ferredoxin is important in the electron transfer cascade required for this nitrogen assimilation. It has been used in studies of assimilation of inorganic nitrogen from nitrite or nitrate in the absence of ammonium.
Ferredoxins are iron-containing proteins that serve as electron-transfer catalysts in photosynthesis and nitrogen fixation.

Packaging

Package sizes are based on ferredoxin content.

Physical form

Contains Tris buffer

Preparation Note

Partially purified by the method of Tagawa and Arnon, Nature, 195, 537 (1962).

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

F3013PROC:
F3013-5MG-PW:
F3013-BULK-N:
F3013-1MG:
F3013-BULK:
F3013-5MG:
F3013-10MG-PW:
F3013-VAR:
F3013-1MG-PW:
F3013-10MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar
Khalifa SAM, et al.
Parasite (Paris, France), 13(1), 51-58 (2006)
Structure of the Mutant E92K of [2Fe-2S] Ferredoxin I from Spinacia oleracea at 1.7 AA Resolution
Binda C, et al
Acta Crystallographica Section D, Biological Crystallography, 54(6), 1353-1358 (1998)
Keita Miyake et al.
The FEBS journal, 287(18), 4016-4031 (2020-01-30)
Bilin pigments play important roles for both light perception and harvesting in cyanobacteria by binding to cyanobacteriochromes (CBCRs) and phycobilisomes (PBS), respectively. Among various cyanobacteria, Acaryochloris marina MBIC 11017 (A. marina 11017) exceptionally uses chlorophyll d as the main photosynthetic pigment absorbing
Harnessing a P450 fatty acid decarboxylase from Macrococcus caseolyticus for microbial biosynthesis of odd chain terminal alkenes
Lee JW, et al.
Metabolic Engineering Communications, 7, e00076-e00076 (2018)
Basilio Zafrilla et al.
Biochemical Society transactions, 39(6), 1844-1848 (2011-11-23)
In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO₃⁻/NO₂⁻ reductase pathway. The small acidic ferredoxin Hv-Fd plays an essential role in
View All Related Papers

Protocols

Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

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