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FLAGIPT1

Millipore

FLAG® Immunoprecipitation Kit

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

usage

 kit sufficient for 50 reactions

Quality Level

300

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

−20°C

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3xFLAG™ peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • A2220ANTI-FLAG M2 affinity gel 1 mLSDS
  • SAE01943X FLAG peptide 1 mgSDS

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000162028
0000162024
128M4163V
019M4046V
019M4047V

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