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Safety Information

FLE250

Sigma-Aldrich

Firefly Lantern Extract

for ATP determination

Synonym(s):

FLE

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.81

Quality Level

200

form

solution

packaging

pkg of 250 mg dried lanterns

technique(s)

toxicology assay: suitable

storage temp.

−20°C

Related Categories

General description

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.

Application

Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.

Biochem/physiol Actions

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.

Other Notes

Crude luciferin and luciferase

Reconstitution

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

FLE250-1VL:
FLE250-BULK:
FLE250-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBX5014
SLBT4266
SLBG6458V
SLBG6458
101M7402

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