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G5160

Sigma-Aldrich

β-Galactosidase from Aspergillus oryzae

≥8.0 units/mg solid

Synonym(s):

β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae

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About This Item

CAS Number:
9031-11-2
Enzyme Commission number:
3.2.1.23 (BRENDA, IUBMB)
EC Number:
232-864-1
MDL number:
MFCD00130623
UNSPSC Code:
12352204
NACRES:
NA.54

form

solid

Quality Level

200

specific activity

≥8.0 units/mg solid

contains

dextrin as extender

storage temp.

−20°C

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General description

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.

Application

β-Galactosidase from Aspergillus oryzae has been used:
  • in in vitro nuclear magnet resonance (NMR)
  • as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
  • to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
  • for enzymatic hydrolysis of lactose
  • for reversed-phase (RP) adsorption
  • in the hydrolysis of whey lactose

Biochem/physiol Actions

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Unit Definition

One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.

inhibitor

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G5160-25KU:
G5160-BULK:
G5160-VAR:
G5160-125KU:
G5160-500KU:

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Certificates of Analysis (COA)

Lot/Batch Number
0000392063
0000379996
0000379997
0000392063
0000379997

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