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I5131

Sigma-Aldrich

IgG from sheep serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym(s):

Sheep IgG

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About This Item

MDL number:
MFCD00165834
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

sheep

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

grade

reagent grade

Assay

≥95% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

isotype

IgG

solubility

saline: 1 mg/mL at -20 °C (Repeated freezing and thawing is not recommended.)

storage temp.

2-8°C

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General description

IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds.

Application

Purified IgG may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, immunoprecipitation and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid-phase immunoadsorbents.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
IgG mediates transplacental transport and participates in the secondary immune response. IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be linked to allergy. It has the longest serum half life.

Analysis Note

Protein concentration should be determined after reconstitution by absorbance at 280 nm (E1%/280 = 14).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

I5131PROC:
I5131-BULK:
I5131-CP:
I5131-10MG:
I5131-100MG:
I5131-50MG:
I5131-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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