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L7759

Sigma-Aldrich

Lectin from Arachis hypogaea (peanut)

peroxidase conjugate, lyophilized powder

Synonym(s):

PNA, Peanut agglutinin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.54

biological source

Arachis hypogaea (peanut)

Quality Level

200

conjugate

peroxidase conjugate

form

lyophilized powder

peroxidase activity

≥20 units/mg protein

technique(s)

western blot: suitable

storage temp.

−20°C

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Application

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

Unit Definition

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Physical form

Contains sodium citrate

Preparation Note

Prepared from peroxidase (P8375) using a modification of a published method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

L7759-.5MG:
L7759-VAR:
L7759-250UG:
L7759-1MG:
L7759-250UG-PW:
L7759-BULK:
L7759-1MG-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
0000404689
0000370511
0000370511
0000325611
0000325608

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