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M8537

Sigma-Aldrich

MCDB 131 Medium

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Product Name

MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

concentration

11.6 g/L

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

General description

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). 

Application

MCDB-131 Medium has been used:

  • as a component of the basal medium to culture bovine retinal endothelial cells
  • to culture bovine lung microvascular endothelial cells
  • for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells

Quantity

Formulated to contain 11.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.18 g/L sodium bicarbonate.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

M8537-10L:
M8537-10X1L:
M8537-50KG:
M8537-PROC:
M8537-ALC2:
M8537LF1-BIBC:
M8537-25KG:
M8537-1L:
M8537-VAR:
M8537-BULK:
M8537-2X5L:
M8537-25K:
M8537-50K:
M8537-1L-KC:
M8537-50L:
M8537-ALC:
M8537-BIBC:

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Certificates of Analysis (COA)

Lot/Batch Number
0000338757
0000338759
0000338761
0000338438
0000338761

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