MAK040

Adipogenesis Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Triglyceride Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.25

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• detection method: colorimetric; fluorometric
• relevant disease(s): endocrinological disorders, diabetes; cardiovascular diseases; cancer
• storage temp.: −20°C

Description

General description

Adipogenesis is the differentiation of pre-adipocytes derived from pluripotent mesenchymal cells into mature adipocytes. Adipocytes, which are capable of storing large quantities of lipids as triglycerides, are the main cells for lipid storage in animals. Adipose tissue plays a critical role in the regulation of whole-body energy homeostasis and adipocyte dysfunction has been implicated in pathological states such as cancer, type II diabetes, cardiovascular disease, and atherosclerosis.

Omental adipocytes are known to promote the invasion and progression of cancer cells especially, ovarian cancer. Close proximity of tumor cells and adipocytes, causes a phenotypic change resulting in the formation of adipocyte-derived fibroblasts, which directly promotes tumor progression. Elevated level of adiponectin produced by adipocytes is observed in heart failure patients, cardiovascular disease, and atherosclerosis. Excess cytokines such as TNFα (tumor necrosis factor -α) in adipocytes results in chronic inflammatory state and inhibits the process of adipogenesis. Adipocytes are known to maintain glucose homeostasis. Adipocytes regulates the entry of fatty acids into circulation and skeletal muscle. This action of adipocytes is associated with the development of type 2 diabetes, which indicates insulin resistance as a result of high levels of fatty acids in the blood.

Application

Adipogenesis Kit has been used to study the intramyocellular triglyceride accumulation in myotubes. It has been used to study the effect of NS5ABP37 on hepatocellular carcinoma by determining the total intracellular triglyceride.

Suitability

Suitable for the quantification of triglyceride accumulation in cells and tissue

Principle

In this kit, total cellular and tissue concentrations of triglycerides are determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the triglycerides present. This kit is able to detect triglycerides in as few as 1 × 10⁴ differentiated 3T3 L1 cells.

Safety Information

• Pictograms: GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H317,H318,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup