MAK555

Lysyl Oxidase (LOX) Assay Kit

sufficient for 500 fluorometric tests

• UNSPSC Code: 41116104
• NACRES: NA.84

Properties

• detection method: fluorometric
• relevant disease(s): dermatological diseases; orthopedic diseases; pulmonary disorders
• storage temp.: −20°C

Description

General description

Lysyl oxidase (LOX) is an extracellular enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. These aldehydes are highly reactive and undergo spontaneous chemical reactions with other lysyl oxidasederived aldehyde residues, or with unmodified lysine residues. This results in cross-linking collagen and elastin which is essential for stabilization of collagen fibrils and for the integrity and elasticity of mature elastin. Lysyl oxidase activity in biological samples is traditionally and most reliably assessed by tritium release end-point assays using radiolabeled collagen or elastin substrates involving laborious vacuum distillation of the released tritiated water. ​

The Lysyl Oxidase Assay Kit offers a sensitive fluorescent assay to measure LOX activity using a LOX substrate that releases hydrogen peroxide upon oxidation. The amount of hydrogen peroxide released by the LOX oxidation is detected using HRP substrate in the HRP-coupled reactions. This allows the detection of sub ng/mL lysyl oxidase. This method eliminates the interference that occurs in some biological samples and can be readily used to detect lysyl oxidase activity in cell culture samples.

Other Notes

Please note that the kit does not include the lysyl oxidase enzyme.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H302,H317,H334,H360FD
• Precautionary Statements: P202 - P261 - P280 - P301 + P312 - P302 + P352 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup